The protein protocols handbook springer protocols handbooks

Advertisement Hide. This service is more advanced with JavaScript available. The Protein Protocols Handbook. Editors view affiliations John M. Front Matter Pages i-xviii. Front Matter Pages Protein Determination by UV Absorption. Pages The Lowry Method for Protein Quantitation.

Thomas D. Friedrich, F. Andrew Ray, Ralph L. Smith, John M. Friedrich, Ralph L. Protein Solubility in Two-Dimensional Electrophoresis.

Brian B. Vandahl, Gunna Christiansen, Svend Birkelund. Anthony G. Subcellular Fractionation of Small Sample Amounts. Page 1 Navigate to page number of About this book Introduction Since the publication of the bestselling second edition of John Walker's widely acclaimed Protein Protocols Handbook , there have been continual methodological developments in the field of protein chemistry. Antibodies Electrophoresis Post-translational modifications Protein blotting Protein function Translation proteins.

Kinetic Silver Staining of Proteins. Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins. Page 1 Navigate to page number of 9. This greatly enhanced second edition introduces 60 critically important new chapters dealing with topics and techniques that have emerged since the first edition, as well as significantly updating the remaining articles. The new chapters cover many rapidly developing areas, particularly the application of mass spectrometry in protein characterization, as well as the now well-established 2-D PAGE technique in proteomics.

The section on glycoprotein analysis has also been significantly expanded, and methods for the production of single-chain and phage-displayed antibodies have been added to the section on antibodies. The authors are commonly the techniques' originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity. Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Second Edition is today's indispensable benchtop manual and guide-not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armametarium of techniques in the urgent search for rapid and still more robust results.

Editors and affiliations. John M. Walker 1 1. University of Hertfordshire Hatfield UK. Reviews "the breadth of techniques covered is large and this alone makes the book of practical value.